Translation of Enzymically Decapped Messenger RNA

964 Transcription and Translation of / ~ Messenger Rna

Messenger R N A that code for the /x chain of secreted (s) and cell surface (m) immunoglobul in M (IgM) differ in size ~ m R N A are 2.4 kilobases [kb]; ~m m R N A are 2.7 kb) and in sequence at their 3' termini (1-4). These sequences predict two different carboxy terminal amino acid sequences and account for peptide (5-7) as well as hydrophobici ty (8, 9) differences that have been shown for t...

Cell-free translation of paramyxovirus messenger RNA.

Polypeptides corresponding in electrophoretic mobility to virion polypeptides 1, 3, and 5 were made in a reticulocyte cell-free system to which 18S RNA from Sendai virus-infected cells was added. Immune precipitation was used to select relevant polypeptides from endogenous products. The cell-free product corresponding to virion polypeptide 3 (the nucleocapsid structure unit) was the most abunda...

-methyladenosine Modulates Messenger RNA Translation Efficiency

N6-methyladenosine Modulates Messenger RNA Translation Efficiency

N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognizes m(6)A and reduces the stability of target transcripts. Looking at additional...

Interferon messenger RNA: translation in heterologous cells.

A viral inhibitor with the characteristics of mouse interferon is produced by avian and simian cells preincubated with RNA extracted from interferon-producing mouse cells. Similarly, RNA extracted from interferon-producing monkey cells induces a monkey interferon-like substance in avian cells and also in a line of simian cells, VERO, which normally lacks the capacity to produce its own interfer...